Arginine methylation in subunits of mammalian pre-mRNA cleavage factor I

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FIGURE 3.
FIGURE 3.

CF Im59 and CF Im68 subunits are differentially dimethylated. Nuclear extract (NE) or cytoplasmic (CE) extract, purified (CF Im purif) recombinant P. pastoris expressed CF Im (rec CF Im) or partially purified CF IIm (CF IIm part) fractions separated on SDS gels and blotted to NC filters were probed with an α-CF Im antibody (reacts with both CF Im59 and CF Im68 subunits) or α-monomethyl, sym10, or asym24 antibody. The label CF Im68/L1 points to CF Im68 (lower band) and CF Im68_L1 (upper band).

This Article

  1. RNA 16: 1646-1659