Mitigated response of endogenous genes to MIR155 is mediated in part by alternate transcript structure. (A) Comparison of relative expression at the whole locus level, the terminal exon level, and in 3′ UTR assays. The genes depicted are only those showing greater than 0.5 RPKMs in whole locus sequencing and in the terminal exon analysis. Error bars are standard error of change. (B) Cumulative distribution plot of terminal exon analysis shows a highly significant relative expression shift for genes with MIR155 seeds versus genes without seeds. P-values as determined by a one-sided Wilcoxon test (Wil.) and a one-sided Kologorov–Smirnov test (K-S) were found to be <2.2 × 10−16. (C) Terminal exon analysis shows significantly more down-regulation than whole locus analysis implicating transcript structure in mitigating MIR155 responses at the whole locus level. P-values were determined by a one-sided Wil. test and a one-sided K-S test.
