Isoform-dependent regulation of TAF5L by MIR155. (A) Gene structures of annotated TAF5L transcripts. (B) Expanded depiction of genomic sequences spanning exons 4 and 5 with accompanying read evidence. Splicing evidence indicates that reads spanning the exon 4/5 junction were found in control (CNTL-1) and MIR155 (155-2)–expressing cells. Pile-ups are a representation of the number of reads identified at each nucleotide position. (C) Quantitative analysis of read coverage (RPKMs) across exon 5 and the unique 3′ UTR sequences containing MIR155 seed sequences. RPKM calculations were performed based on the number of reads in control Mutu I cells. (D) Relative expression of TAF5L whole locus (W.L.), TAF5L exons 4 and 5, and unique 3′ UTR containing MIR155 seed sequences were calculated based on RPKM calculations performed using the appropriate respective feature annotations. Error bars are standard error of change. (E) 3′ UTR assay showing suppression of 3′ UTR containing MIR155 seed sequences. These results are extracted from the data shown in Figure 4.
