DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures

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FIGURE 4.
FIGURE 4.

The dsRBD2 and CTT of DGCR8 retain the ability to bind cooperatively to pri-mir-30a. (A) Filter binding assays of DGCR8590–751. The data were fit using the cooperative trimer model. (B) Hill plot of the data shown in A. Only the region in the binding transition is indicated. (C) Weak but detectable pri-mir-30a processing activity of DGCR8590–751 at a protein concentration above the binding transition.

This Article

  1. Published in Advance June 17, 2010, doi: 10.1261/rna.2111310 RNA 16: 1570-1583

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