Inhibition of HCV IRES translation with pseudoknot-targeted 2′-OMe oligonucleotides. (A) The diagram shows to which regions of the IRES each of the four 2′-OMe oligonucleotides is complementary, with the start site AUG shown in bold. (B) Titrations of the 2′-OMe oligonucleotides in WT IRES-FF luciferase in vitro translation reactions, fit to sigmoidal inhibition curves. Plotted points represent the mean activities of four translation reactions from two independent transcriptions and the error bars extend 1 SD above and below the mean. The 1 μM concentration utilized in toeprinting experiments in C is indicated with a vertical dashed line. (C) Primer extension inhibition and toeprinting on the WT IRES-FF luc mRNA in the presence of 1 μM 2′-OMe oligonucleotides.
