Tailoring the switch from IRES-dependent to 5′-end-dependent translation with the RNase P ribozyme

RNase P recognition of the defective CGCCC IRES induces an increase in reporter translation. (A) Ribozyme-mediated cleavage of the reporter RNA. Real-time RT-qPCR analysis of reporter RNAs in cells transfected with the different versions of the ribozyme fused to the CGCCC IRES. Data are expressed as in Fig. 1B. (B) Time course of reporter translation in response to expression of the rnpB ribozyme, fused to the IRES carrying a CGCCC sequence in the RAAA motif. Data are represented as in Fig. 2A. (C) Changes in reporter levels induced by expression of the RNase P ribozyme. Data are represented as in Fig. 2C.