Tailoring the switch from IRES-dependent to 5′-end-dependent translation with the RNase P ribozyme

RNase P recognition of the defective GUAG IRES leads to increased expression of the reporter. (A) Ribozyme-mediated cleavage of the reporter RNA. Real-time RT-qPCR analysis of reporter RNAs in cells transfected with the different versions of the ribozyme fused to the GUAG IRES. Data are expressed as in Fig. 1B. (B) Time course of reporter translation in response to expression of the rnpB ribozyme, fused to the IRES element carrying the single GUAG mutation in the GNRA motif. Data are represented as in Fig. 2A. (C) Changes in translation levels induced by expression of the RNase P ribozyme. Data are represented as in Fig. 2C.