FIGURE 4.
uAUG recognition. (A) The uAUGs of the AtbZip11 uORFs were fused directly to FLUC while retaining the start codon context up to the +4 position as well as their distance from the transcription start site as shown. Note that all uAUGs appear to be recognized in an eIF3h-independent fashion (n = 7–14). Experimental conditions are as in Figure 2. (B) uAUG-FLUC fusion constructs to examine the (re)initiation potential at individual uAUGs downstream from uORF1. Bars, standard error (n = 5–6). (*) Statistical significance at P < 0.02.
