FIGURE 1.
In eif3h mutant plants, 43S complexes lack eIF3h. (A) Wild-type eIF3h and truncated eIF3h expressed in eif3h-1 mutant plants were visualized by immunoblotting with anti-eIF3h antibody. (*) Crossreacting protein. (B) Sucrose gradients from wild-type and eif3h mutant plants were examined for 18S ribosomal RNAs to identify 40S subunits using electrophoresis and ethidium bromide staining. Corresponding protein extracts were examined for eIF3 subunits by SDS-PAGE and immunoblotting. (Arrows) Topmost fractions containing 40S and 60S subunits.
