High-throughput quantification of splicing isoforms

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FIGURE 2.
FIGURE 2.

Systematic tiling of BSPs across an exon–exon junction. BSPs harboring 15, 13, 11, and 9 bp complementarities to BCAS1 long splicing isoform in their 3′ end show amplification curves, while those with 8, 7, 5, and 3 bp or no template controls show no amplification. AIR and DCS stand for alternatively included region and downstream constitutive sequence, respectively.

This Article

  1. Published in Advance December 28, 2009, doi: 10.1261/rna.1877010 RNA 16: 442-449

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