Intended transcriptional silencing with siRNA results in gene repression through sequence-specific off-targeting

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FIGURE 3.
FIGURE 3.

Luciferase assay performed in human cells containing an integrated HIVSF2-LTR promoter driven reporter gene and co-transfected with siRNA targeting the HIVSF2-LTR promoter and Tat expression plasmid, pSV40.Tat-86. (A) Schematic representation of the luciferase reporter plasmids engineered to contain either wild-type (pGL4.20.SF2-LTR-Luc) or mutated (pGL4.20.SF2mut-LTR-Luc) promoter target sequences within the HIVSF2-LTR promoter. Mismatched bases are underlined. (B) Luciferase (firefly) gene activity from HeLa cells containing an integrated reporter gene when treated with siSF2-247 or its variants. Tat-induced firefly luciferase activity is normalized to that from cells not transfected with siRNA (“Mock”) and is presented as the percentage mean ± SD from three independent experiments, with three replicate samples per experiment; (*) p < 0.05.

This Article

  1. Published in Advance December 21, 2009, doi: 10.1261/rna.1808510 RNA 16: 430-441

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