Luciferase assay performed in human cells co-transfected with VEGF promoter containing reporter plasmid and siRNA targeting the VEGF promoter. (A) Schematic representation of the luciferase reporter plasmids engineered to contain either wild-type (pVEGFprom2.3-Luc), mutated (pVEGFprom2.3.VFp992mut-Luc), or deleted (pVEGFprom2.3.VFp992del-Luc) promoter target sequences. Mismatched bases are underlined, and deleted bases are indicated with a hyphen. (B) Luciferase (firefly) gene activity from HeLa cells transfected with each reporter plasmid when treated with siVFp(−992) or its variants. Luciferase activity is normalized to that from cells transfected with vector without siRNA (Vector alone) and is presented as the percentage mean ± SD from three independent experiments, with three replicate samples per experiment; (*) p < 0.05.
