Intended transcriptional silencing with siRNA results in gene repression through sequence-specific off-targeting

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FIGURE 1.
FIGURE 1.

VEGF expression in human cells treated with siRNA targeting the endogenous VEGF promoter. (A) VEGF expression in HeLa and ARPE19 cells, transfected with siRNAs designed to specific sites within the endogenous VEGF gene promoter. (B) VEGF gene expression in HeLa cells transfected with siVFp(−992) and its variants. VEGF expression from cells transfected with each siRNA is normalized to “Mock” transfected cells and presented as the percentage mean ± SD from three independent experiments, with three replicate samples per experiment; (*) p < 0.05.

This Article

  1. RNA 16: 430-441