Anti-Argonaute RIP-Chip shows that miRNA transfections alter global patterns of mRNA recruitment to microribonucleoprotein complexes

MiRNA transfections lead to systematic changes in the mRNAs that are incorporated into AGO-miRNPs as determined by RIP-Chip. The abscissa shows six different groups of mRNAs. Enrichment in the AGO-miRNP increases from left to right according to the P-value of t-tests comparing the mRNA amount in anti-AGO co-IP versus anti-NMS co-IP. mRNA groups were P = 0.1–0.2 (N = 3503 mRNAs), P = 0.05–0.1 (N = 1786), P = 0.01–0.05 (N = 1594), P = 0.001–0.01 (N = 531), P = 0.00001–0.0001 (N = 82), and P < 0.00001 (N = 10). Note that for the group with the greatest enrichment in the AGO-miRNPs at baseline (right-most data point), transfections with miR-107 and miR-128, but not miR-124 or miR-320, cause a decrease in these mRNAs in the AGO-miRNP.