Anti-Argonaute RIP-Chip shows that miRNA transfections alter global patterns of mRNA recruitment to microribonucleoprotein complexes

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FIGURE 2.
FIGURE 2.

Schematic illustration of biochemical identification of miRNA targets using RIP-Chip. miRNPs are co-IPed with anti-AGO (2A8) antibodies (Nelson et al. 2007) bound to protein G-agarose beads. RNAs that associated with AGO protein are processed for Affymetrix Gene 1.0 ST microarrays and this high-throughput assay can resolve miRNA targets.

This Article

  1. Published in Advance December 30, 2009, doi: 10.1261/rna.1905910 RNA 16: 394-404

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