Anti-Argonaute RIP-Chip shows that miRNA transfections alter global patterns of mRNA recruitment to microribonucleoprotein complexes

MicroRNA (miRNA) transfections are effective and lead to specific targeting by miRNAs. All transfections (25 nM) were performed in H4 glioneuronal cells and cells were harvested 48 h after transfection. (A) Northern blot analysis of miRNPs isolated after miRNA transfections with miR-124 and miR-128. The Northern blot analysis showed that miRNAs were specifically recruited to miRNPs after miRNA transfections. (B) Renilla luciferase (Rluc) reporter assays showed that miRNAs are functionally incorporated after miRNA transfections. MiR-107 and miR-320 specifically target reporter constructs bearing 3′-untranslated region recognition sequences for those miRNAs.