Preferential translation of Hsp83 in Leishmania requires a thermosensitive polypyrimidine-rich element in the 3′ UTR and involves scanning of the 5′ UTR

RNA melting curves of the wild-type and mutated 1–472 RNA fragments. Representative melting profiles were obtained by measuring the optical density of the RNA solutions (0.012 mg/mL) at temperatures that increased by increments of 5°C. The RNA was allowed to equilibrate for 10 min at each temperature, prior to monitoring the absorbance at 260 nm. The change in absorbance for each temperature (ΔOD₂₆₀) was calculated relative to the starting point. The ΔOD₂₆₀ values for each curve are expressed as relative numbers that range from 0% to 100% (the ΔOD₂₆₀ at the highest temperature served as 100%).