Expression patterns of lncRNAs, pluripotent markers, trophectoderm markers, and epiblast lineage markers in response to lncRNA overexpression (OE). (A) LncRNAs AK028326 and AK141205 are significantly overexpressed relative to the vector-only control. (B) AK028326 overexpression marginally altered Oct4 and Nanog transcription. (C) AK141205 overexpression significantly up-regulated Oct4 but not Nanog transcription relative to vector-only control. (D) In response to AK028326 overexpression, there was significant enhancement of mesodermal marker T/Brachyury and Sox4, the epiblast marker Fgf5, the ectodermal markers Sox11, Pax6, and Pax7, in addition to unchanged levels of the housekeeping genes Gapdh and Ahcy. (E) In response to AK141205 overexpression, there was significant enhancement of mesodermal marker T/Brachyury, the epiblast marker Fgf5, the ectodermal markers Sox1 and Sox11, and endoderm markers, in addition to unchanged levels of Gapdh and Ahcy. (F–H) AP staining of AK028326-OE (F), AK141205-OE (G), and vector-only control (H). Scale bar, 100 μm. (Asterisks) Significant difference from control samples (*, P < 0.05; ***, P < 0.001) relative to the pCAG control (dotted red line).
