Differential expression of lncRNA upon robust Oct4 and Nanog RNAi knockdown. (A) shRNA-directed knockdown resulted in greater than or ∼80% reduction of Oct4 mRNA and Nanog mRNA, respectively, compared with the non-silencing control (NS control) and the pSUPER-PURO vector-only control (pSUPER-PURO control), 3 d post-transfection. (B) shRNA-directed knockdown resulted in comparable levels of Nanog mRNA reduction and Oct4 mRNA compared with the NS control and the pSUPER-NEO vector-only control (pSUPER-NEO control), 3 d post-transfection. (C) Oct4 RNAi resulted in down-regulation of the two putative Oct4 targets, AK005651 and AK028326. (D) Nanog RNAi resulted in down-regulation of the putative Nanog target AK043754 and up-regulation of AK141205. (Asterisks) Significant difference from control samples (*, P < 0.05; **, P < 0.01) relative to the NS control (dotted black line).
