FIGURE 1.
(A) Secondary structure comparisons of PKF and full-length tmRNA from T. thermophilus. The domain in common, comprising the TLD (tRNA-like domain) and the helix H2, is shown in black. The figure is adapted from the tmRDB website (http://rnp.uthct.edu/rnp/tmRDB/tmRDB.html) (Andersen et al. 2006). (B) Biochemical characterization of the ribosomal complexes. After purification by size exclusion chromatography, ribosome complexes were analyzed by SDS-PAGE. The elongation factor EF-Tu was identified on the silver-stained gel, while SmpB and PKF occupancies were measured by Western blot and radiolabeling, respectively.
