Selections that optimize RNA display in the yeast three-hybrid system

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FIGURE 7.
FIGURE 7.

Comparison of Y3H RNA expression cassettes. (A) Transcript from conventional RNA expression cassette. (B) T-cassette design (Wurster et al. 2009) to enhance modularity (folding autonomy) and accessibility of RNA inserts emanating from a four-way helical junction. (Black dots) Transcript 5′ termini. Transcript 3′ terminal sequences are truncated for clarity. Bacteriophage MS2 hairpins are labeled. (Arrows) Cloning sites.

This Article

  1. RNA 16: 253-258