FIGURE 5.
Anti-p50 Y3H selections and affinity analysis in vitro (Cassiday and Maher 2003). (A) Predicted secondary structure of in vitro selected anti-p50 RNA aptamer (left) compared to predicted secondary structure Y3H-selected variant from early in vitro selection RNA library (right). (B) Comparison of aptamer activities in Y3H assays. 3-AT gradient plate yeast growth image (left) and liquid β-galactosidase assays values (right) compare HIS3 and lacZ reporter gene signals. (C) Binding isotherms for isolated RNA aptamer complexes with p502 showing similar affinities despite different Y3H readouts.
