FIGURE 4.
Steric considerations in the Y3H system. (A) Multiple LexA (black circles)/MS2 coat (gray circles) dimers are bound to yeast promoter DNA near lacZ and HIS3 reporter genes. (B) The RNA insert is displayed in the context of multiple dynamic RNA–protein complexes. Y3H readout reflects the intrinsic affinity of the target protein for the RNA insert, but also contributions of RNA display (RNA tertiary structure and accessibility).
