Theoretical contributions to Y3H readout. Y3H readout reflects the combination of the intrinsic affinity of an isolated RNA insert for a protein target as measured in a biochemical experiment (black), RNA insert tertiary structure in the context of the Y3H transcript (gray), and accessibility of the RNA insert at the reporter gene promoter in vivo (white). (A) Correlation between intrinsic affinity and Y3H readout for RNAs 1–3 because display factors are constant. (B) Lack of correlation between intrinsic target affinity and Y3H readout for RNAs 4–6 because of differing display. (C) Different Y3H readouts for RNAs 7–9 with comparable intrinsic target affinities because of differing display.
