Selections that optimize RNA display in the yeast three-hybrid system

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FIGURE 1.
FIGURE 1.

Schematic illustration of Y3H. (A) The RNA insert (red) is expressed in the context of RNA vector sequences (black) tethered upstream of lacZ (brown) and HIS3 reporter genes via a MS2 coat–LexA fusion protein (blue and black). Gene activation depends on binding of the Gal4 activation domain (yellow) –prey fusion protein (green).

This Article

  1. Published in Advance December 14, 2009, doi: 10.1261/rna.1880410 RNA 16: 253-258

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