FIGURE 5.
Effects of Oct4 knockdown on cell differentiation. H1 cells were transfected with siCon (A,C) or siOct4 (B,D). Cells were analyzed by phase contrast microscopy (A,B, 200× magnification) or stained for alkaline phosphatase expression (C,D, 40× magnification). (C,D) Cell colonies were outlined with arrows pointing to the center of the colonies. As differentiated cells had enlarged cell size and increased cytoplasmic area, the colonies thus appeared larger and fused together with each other.
