High-efficiency siRNA-based gene knockdown in human embryonic stem cells

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FIGURE 4.
FIGURE 4.

siRNA knockdown of Oct4 in H1 and H7 cells. (A,C,E,G) Results of qRT-PCR. Relative mRNA levels are shown after normalization against β-tubulin mRNA. mRNA levels in cells transfected with siCon were arbitrarily set as 1. Numbers are mean ±SD (n = 3). (B,D,F,H) Representative Western blot results. Antibodies used are shown on the left. Oct4 protein levels in cells transfected with siCon were arbitrarily set as 100%. (A–F) Results from H1 transfection. (G,H) results from H7 transfection.

This Article

  1. Published in Advance October 26, 2010, doi: 10.1261/rna.2350710 RNA 16: 2564-2569

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