FIGURE 4.
Kinetics of silencing, siRNA tissue uptake, and Ago2 loading in mouse liver. Mice were treated with a single intravenous dose (3.6 mg/kg) of LNP201b-Ssb siRNA and harvested at the indicated time points. (A) Silencing of the Ssb target mRNA. Expression levels of Ssb relative to housekeeping Ppib were determined by quantitative RT-PCR and expressed as percentage values in relation to PBS-treated animals. (B) Normalized quantities of Ago2-assocaited siRNA guide and passenger strands. (C) Normalized amounts of siRNA guide and passenger strands in liver homogenates.
