Characterization of Ago-specific antibodies. (A) Specificity of the antibodies for immunoprecipitation of overexpressed Ago proteins. Indicated rodent and human Ago proteins with N-terminal Flag-HA tags were transiently expressed in HEK 293T cells, immunoprecipitated with the indicated antibodies, and detected in Western blot using an anti-HA antibody. (B) Immunoprecipitation effectiveness of the antibodies using either mouse or rhesus monkey total liver lysates. Lysates (30 μg) were loaded as input (5%). Antibodies used for Western blot after immunoprecipitation are indicated to the right of individual blots. The species specificity of the antibodies is represented with the abbreviation (m, mouse; h, human/monkey) before the protein name. Solid arrowhead, hollow arrowhead, and asterisk denote Ago2, Ago1, and Ago protein members, respectively.
