FIGURE 5.
Effect of the down-regulation of hAgos on siRNA-based gene regulation. A day after the transfection of ECV304 cells with 100 nM siAgo (A) or asON (B), a second transfection was performed with 100 pM siLam. Then, 48 h after the first transfection, cells were collected, the total RNA was isolated, reverse transcribed, and the cDNA was quantified by RT–qPCR. Values of the housekeeping gene β glucuronidase were used for the normalization of the data. As controls, asON_Ctr and siscr were used and set at 1. Indicated are mean values ±SEM (n = 4-6).
