FIGURE 2.
The asONs show a high selectivity toward their target mRNA. ECV304 cells were transfected with 100 nM asON, then 24 h after transfection, cells were collected and the total RNA was isolated and reverse transcribed. The cDNA was quantified by RT–qPCR and hAgo-specific standard curves were used for absolute quantification of hAgo mRNA. Indicated are copies of hAgo mRNA per nanogram total RNA. As control, asON_Ctr was used, which has no endogenous target, and a fully modified phosphorothioate backbone. Indicated are mean values and standard deviations.
