TbRGG2 facilitates kinetoplastid RNA editing initiation and progression past intrinsic pause sites

TbRGG2 can unwind RNA. (A) E. coli strain RL211 contains the trpL terminator that forms a hairpin loop upstream of a chloramphenicol (chl) resistance reporter gene (CAT). (B) RL211 cells expressing empty pINIII vector, CspE (positive control), or TbRGG2 were grown in LB with 100 μg/mL ampicillin and 1 mM IPTG for 1 h to an approximate OD₆₀₀ of 1 and spotted on LB plates containing 50 μg/mL ampicillin and 1 mM IPTG, with and without 15 μg/mL chl. Cells were grown at 37°C for 1 d (– chl) or 4 d (+ chl).