TbRGG2 facilitates kinetoplastid RNA editing initiation and progression past intrinsic pause sites

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FIGURE 7.
FIGURE 7.

Recombinant TbRGG2 stimulates annealing of A6 pre-mRNA to cognate gRNA in vitro. (A) Representative annealing assay with radiolabeled A6U5 41nt mRNA and gA6[14]NX gRNA at the indicated protein concentrations. (Lane 1) A6U5 pre-mRNA alone, (lane 2) A6U5 mRNA and gA6[14]NX gRNA in the absence of protein, (lanes 3–7) cognate gRNA/mRNA pair in the presence of increasing TbRGG2, (lane 8) RBP16 positive control, (lane 9) p22 negative control, (lane 10) reaction with TbRGG2 and a noncognate gRNA/mRNA pair. (B) The bar graph represents the mean and SE of the percentage of the input pre-mRNA that is annealed to the gRNA in at least three separate experiments.

This Article

  1. Published in Advance September 20, 2010, doi: 10.1261/rna.2285510 RNA 16: 2239-2251

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