TbRGG2 facilitates kinetoplastid RNA editing initiation and progression past intrinsic pause sites

Depletion of TbRGG2 results in an increased pool of partially edited RNAs. Gel analysis of RT-PCR reactions using RNAs from TbRGG2 RNAi cells that were induced (I) and uninduced (UI) for 72 h. Primers to the RPS12 and A6 genes are specific to nucleotides outside of the edited sequence and amplify the entire population including unedited (UE), partially edited (PE), and fully edited (FE) RNAs. Mitochondrial (Mito) DNA was used as a template control for sizing the unedited RPS12 transcript.