
Depletion of TbRGG2 does not affect total gRNA levels. Guanylyltransferase labeling of gRNAs from the PF TbRGG2 RNAi cells at 72 h with or without tetracycline induction. Twenty micrograms of total RNA was labeled with [α-32P] GTP using guanylyltransferase and resolved on a denaturing gel. RNA input was standardized against the quantity of labeled cytoplasmic RNA.










