Metal binding and substrate positioning by evolutionarily invariant U6 sequences in catalytically active protein-free snRNAs

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FIGURE 7.
FIGURE 7.

The snRNA-catalyzed splicing reaction is pH dependent. (A) The efficiency of product formation increases with rising the pH from 7.0 to 8.8. The arrow points to the product. Location of the Exon1 precursor is shown. (B) Reaction rate increases as pH is changed from 8.0 to 8.8. (C) Second-order rate constant for product formation from pH 8 to 8.8.

This Article

  1. Published in Advance September 8, 2010, doi: 10.1261/rna.2170910 RNA 16: 2226-2238

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