Chemical probing in-line experiments: Oligonucleotide complexes, in which the mRNA is labeled (Materials and Methods), were mixed and incubated as described in the Materials and Methods for 40 h at room temperature. The reactions were then mixed with a stop solution that contains 8 M urea and dyes, analyzed in a 10% gel, and autoradiographed. RNA was labeled as described in the Materials and Methods. The figure shows the nucleotide size of markers on the lefthand side. The lanes are grouped under the mRNA that was used in the complex. Alk indicates alkaline digest of CAT mRNA; T1, partial RNase T1 digest of CAT mRNA; “−” no added EGS; E-wild type CATEGS, P-CATPMO EGS; and U, unincubated mRNA.
