Morpholino oligonucleotides do not participate perfectly in standard Watson-Crick complexes with RNA

Inhibition of EGSs on CAT EGS* and CAT PP* mRNAs. Conditions were the same as used in Figure 4 except that the mRNAs were EGS* and PP*. Concentrations of particular EGSs are given in Figure 1. (A) EGS varied with CAT^PMO held constant with EGS* substrate. (B) CAT^PMO varied and CAT^EGS constant with CAT EGS* substrate. (C) EGS constant with CAT^PPMO varied with PP*substrate. (D) EGS constant with CAT^PMO varied with PP* substrate. (A, B) Cleavage occurs at the PP site. (C, D) Cleavage occurs at the EGS site.