Morpholino oligonucleotides do not participate perfectly in standard Watson-Crick complexes with RNA

Cleavage assays for the EGS* and PP* mRNA mutants. The conditions are the same as for Figure 1 except the mutant substrates were used as indicated with wild-type EGS (A), CAT^PPMO (B), CAT^PMO (C), and EGS (D) used in the reactions. (A–C) Substrate CAT EGS* (cleavage at the PP site). (D) Substrate CAT PP* (cleavage at the EGS site). The labels at the top of each graph indicate which substrate was used, and that is followed by which particular EGS was employed, as written in the legend to Figure 1.