Morpholino oligonucleotides do not participate perfectly in standard Watson-Crick complexes with RNA

Amount of RNase P cleavage as a function of time. (A) CAT^EGS; (B) CAT^PPMO; (C) CAT^PMO. The methods for assay are in Materials and Methods. The amount of labeled mRNA (CAT mRNA) was 0.01 μM and was used as substrate; EGS oligonucleotide, 0.5 μM (50×); PPMO, 2 μM (200×); and PPMO, 0.2 μM (20×). At the top of this figure and the others, both the mRNA and the EGS used are listed as is appropriate. The experimental error is ±5% for each point.