Coupled RNA polymerase II transcription and 3′ end formation with yeast whole-cell extracts

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 4.
FIGURE 4.

Analysis of rat1-1 extracts in coupled in vitro transcription/processing. (A) Time-course of in vitro transcription/processing with rat1-1 mutant extracts with templates carrying wild-type CYC1 (lanes 1–5) and mutant cyc1-512 (lanes 6–10) terminators, as indicated. (B) Quantification of results obtained in A. (C) 3′ end analysis as outlined in Figure 3D, of time-course obtained with rat1-1 in vitro transcription/processing reactions. Minus reverse transcriptase (−RT) controls were included for 40 min time points (lanes 5,10).

This Article

  1. Published in Advance September 1, 2010, doi: 10.1261/rna.2172510 RNA 16: 2205-2217

Current Issue

  1. August 2026, 32 (8)
  1. Current Issue
  1. Alert me to new issues of RNA