FIGURE 6.
Endogenous xPat1a is a component of the CPEB complex, not endogenous xPat1b. (A) xPat1a and xPat1b coelute with CPEB in a large complex of ∼3 M Da in a Superose 6 HR10/30 gel filtration column loaded with stage V/VI oocyte lysate. Column fractions were analyzed by Western blotting, with the indicated antibodies. (B) CPEB antibody coimmunoprecipitates endogenous xPat1a but not xPat1b in oocytes. (C) xPat1a antibody coimmunoprecipitates CPEB, whereas anti-xPat1b antibody does not. (D) NRAS RNA G-quadruplex interacts with the CPEB repression complex. Wild-type biotinylated NRAS RNA G-quadruplex interacts preferentially with components of the CPEB complex as compared with a biotinylated mutant sequence.
