FIGURE 5.
Intragenic transcription promotes full-length ASP3 gene expression in cis but not in trans. (A) Northern analysis of the single copy–ASP3 wild-type and iceΔ strains in ammonium-containing medium and under nitrogen starvation. The ASP3 and ncASP3 signals were normalized to that of ACT1 and are shown below as the average of three independent experiments. (B) Total RNAs were extracted from the wild-type strain containing the empty vector or ncASP3-expressing plasmid under the control of the ADH1 promoter in ammonium-containing medium and under nitrogen starvation. Following Northern blotting, the ASP3 and ncASP3 signals were normalized to the signal of ACT1. Values are displayed below the blots.
