FIGURE 3.
Characterization of the RlmH-directed methylation. Determination of catalytic parameters of native RlmH for substrate 70S ribosomes (top panel) and cofactor S-adenosyl-L-methionine (SAM) (bottom panel). The initial rate of methylation was measured in the presence of 84 nM purified RlmH, 100 μM [3H]-SAM and increasing concentrations of ΔrlmH 70S ribosomes, or increasing concentrations of [3H]-SAM and 2 μM ΔrlmH 70S ribosomes, respectively. The amount of [3H]-methyl groups incorporated was monitored by acid precipitation and scintillation counting. One experiment representative of three repeats is shown.
