Specificity and kinetics of 23S rRNA modification enzymes RlmH and RluD

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FIGURE 2.
FIGURE 2.

Association of 30S and 50S subunits is necessary for RlmH activity. ΔrlmH 70S ribosomes (filled circles and solid line) or 50S and 30S subunits (open circles and dotted line) were methylated at various Mg2+ conditions using [3H]-SAM and purified RlmH as described in Materials and Methods. Incorporation of [3H]-methyl groups into TCA insoluble matter was determined. One experiment representative of three repeats is shown.

This Article

  1. Published in Advance September 3, 2010, doi: 10.1261/rna.2234310 RNA 16: 2075-2084

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