FIGURE 5.
miR-21 targeting of endogenous PDCD4 is ablated when active cellular PABP is increased. 293T cells were transfected with control or Paip2 siRNAs and then transfected with miR-21 or miR-30 ∼20 h later (left). After another 20 h incubation, cells were lysed and protein levels evaluated by Western blot. Effects of PABP overexpression were similarly evaluated (right). Quantification of PDCD4 normalized to tubulin levels in Western blots is shown (lower panels).
