FIGURE 3.
miR-155 represses cap- and IRES-driven targets containing the authentic BACH1 3′ UTR. (A) Schematic of reporter constructs containing the BACH1 3′ UTR with four predicted miR-155 sites (black ovals). The sequence of mature miR-155 is shown with the seed sequence in capital letters. (B) Expression of miR-155 was evaluated in 293T cells by primer extension. (C) miR-155 repression levels of the indicated constructs. (D) qRT-PCR (upper panel) and Northern blot (lower panel) analyses were performed. (*) Background band consistently observed with the nontarget control.
