SHQ1 is required prior to NAF1 for assembly of H/ACA small nucleolar and telomerase RNPs

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FIGURE 2.
FIGURE 2.

In vivo, SHQ1 only binds NAP57 alone. Indirect immunofluorescence of transfected LacI-SHQ1 constructs tethered to lac operator repeats stably integrated into the genome of U2OS cells (arrows) and triple labeled for LacI (panel 1), the indicated endogenous H/ACA RNP associated factors (panel 2), and by fluorescent in situ hybridization for simultaneously four H/ACA RNAs, E3, ACA8, ACA18, and ACA25 (panel 3). Note, LacI-SHQ1 only recruits NAP57 (A) but none of the other H/ACA associated proteins (D–G) nor RNAs (A, panel 3). The NAP57 recruitment excludes the CS domain of SHQ1 (B) and is confined to its C-terminal SHQ1-ΔCS domain (C). Bar, 10 μm.

This Article

  1. Published in Advance April 21, 2009, doi: 10.1261/rna.1532109 RNA 15: 1188-1197

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