FIGURE 8.
Functional analysis of the long (pRF-L) and short (pRF-S) CIRP 5′-UTRs in response to hypoxia by transient transfection. NIH-3T3 cells transfected as described in Figure 4 were grown under either normoxic (gray) or hypoxic (black) conditions (1% O2) at 37°C and assayed for luciferase activity 24 h post-transfection. The ration of firefly to renilla luciferase expression is shown after normalization to control β-galactosidase expression. All data shown are the means of triplicate experiments ± SD. For full details see the text.
