Conservation of protein sequences near the amino acid in the β carboxyltransferase subunit of ACCase affected by editing of C794. Alignment of β carboxyltransferase protein sequences from nine plant and one bacterial species. Conserved regions are boxed. Alignment was performed by T-COFFEE and viewed with GeneDoc. GenBank accessions containing the accD gene from each species used are: AP000423, DQ345959, EF489041, DQ317523, X56315, DQ424856, Z00044, AM087200, AC093544, and NC_000913. Upper panel: amino acid sequences of the central conserved region containing the residue affected by RNA editing (arrow). Conserved cysteinyl zinc ligands (Bilder et al. 2006) are indicated by (z). Numbers indicate residues in the Arabidopsis sequence. Lower panel: graphical depiction of ACCase β carboxyltransferase subunit protein alignment. An enlarged version of the complete alignment can be viewed in Supplemental Figure S5. The conserved carboxyl transferase domain is underlined; arrow and z symbols are as in the upper panel.
